The Wallaroos have been outmuscled by the USA, going down 33-12 in Kansas City.
It came after the match was delayed for over 2.5 hours due to the weather, with the Wallaoros dealing with a tornado warning during the week.
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However, they couldn't manage the storm on the field as the power of the Eagles proved too hard to handle, securing the win with two late tries as the clock went well past midnight in Missouri.
"Definitely disappointed in the result. We can definitely say that ill-discipline was the reason why we probably weren't able to play the rugby game that we wanted," captain Siokapesi Palu Sekona said post-match.
"Playing a host nation, they're always going to have an advantage in that space, but there's no excuse from our end.
"We've always been an us-versus-us mentality, and what it means is that we've got a lot of work to do once we go back. We'll travel home and start working towards that match."
The Australians were on the back four for the majority of the first half, making over 120 tackles and finding themselves on the wrong end of a 9-2 penalty count.
It forced some desperate defence as they sought to hold off the powerful American pack for as long as possible.
Prop Hope Rogers eventually crossed for the opener from close, her second in as many games.
The penalty count proved costly, with Maya Stewart finding herself sitting down for ten after 15 minutes amid back-to-back penalties.
But a smart 50-22 from Samantha Wood allowed the chance for a rare counterattack, with Palu Sedona crashing over for the Aussies.
The Americans eventually restored their lead through the excellent Georgie Perris-Redding, stretching out for a 14-7 lead after 28 minutes.
Wallaroos hooker Tania Naden thought she’d hit straight back; however, a knock-on in the prior maul denied her a five-pointer on her return to Test Rugby.
But Desiree Miller couldn’t be denied as she crossed to start the second half to reduce the lead to two.
Miller combined nicely with Stewart, who set up the field position with a break seconds into the second half.
However, it failed to change the tide of the match as the Americans’ physicality was too much to handle with the game bleeding into Saturday (local time.)
Freda Tafuna hit right back for the Eagles, continuing her love affair with playing the Aussies following her hat-trick in last year’s draw.
Rogers made it two with 11 minutes to go to seal the victory, with the prop denied earlier before crashing over a metre from the line.
The Aussies tried to chase the game late, but ultimately, there was no way through as the Eagles came away with the deserved win.
